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Selina Keppler - Inflammation and Immunity

Research focus

B cells can influence local and systemic immunity by antigen presentation, cytokine secretion and antibody production. The research of the Keppler lab focusses on the role of B cells in autoimmune disorders, using a mouse model of Wiskott-Aldrich Syndrome. We are especially interested in the crosstalk of B cells with specialized inflammatory niches during autoimmunity, such as the gut or adipose tissue. We developed EMOVI (easy ECi clearing and multi-organ volumetric imaging) - a 3D whole mount imaging approach to reveal cell-cell as well as cell-niche interactions in unprocessed tissue. In order to understand the complexity of autoimmune processes we combine our imaging approach with high-parametric flow cytometry, mass cytometry (CyTOF) and in vitro culture systems to define drivers of inflammation during homeostatic and inflammatory conditions. Furthermore, we established collaborations with physicists and microscopists (e.g. AG Pleitez) to develop new approaches in order to achieve a more systemic analysis of autoimmune disorders. Mechanistically, we study the influence of the actin cytoskeleton on signalling pathways and organelle biogenesis during B cell metabolism, differentiation and function.

We are enthusiastic about our work and enjoy teaching students on our topics and techniques. Likewise, we thrive on collaborative projects and are looking forward to many future interactions within and outside the TranslaTUM.

Selina studied Biology with a focus on Molecular Immunology, Virology and Microbiology at the University of Freiburg and the ENS Lyon; obtaining her diploma degree in 2007. She was then awarded a postgraduate fellowship from the Boehringer Ingelheim Fonds (BIF) to conduct her doctoral studies in Immunology, investigating the role of cytokines in T cell activation and memory formation during infection. After receiving her Dr. rer. nat in 2011, Selina joined the group of Facundo D. Batista at Cancer Research UK (now the Francis Crick Institute) in London, where she was awarded a fellowship of the German Research Foundation (DFG) to study the role of actin regulators in mediating B cell receptor signaling. In 2017, Selina was awarded funding from the DFG to investigate the role of the actin cytoskeleton in mediating receptor signaling to regulate B cell responses during inflammatory challenges at the TranslaTUM in Munich. In 2019, Selina was awarded funding from the Else-Kröner-Fresenius Stiftung to follow her research interest of the role of B cells during Inflammatory Bowel Disease.

  • Else-Kröner-Fresenius Stiftung (2019) – Research grant
  • German Research Foundation (DFG) (2017)   – Research grant
  • German Research Foundation (DFG) (2011)   – Research Fellowship
  • Boehringer Ingelheim Fonds (BIF) (2007) - PhD Fellowship

Fan, H., Sievert, W., Hofmann, J., Keppler, S.J., Steiger, K., Puig-Bosch, X., Haller, B., Rammes, G., and Multhoff, G. (2022). Partial-Brain Radiation-Induced Microvascular Cognitive Impairment in Juvenile Murine Unilateral Hippocampal Synaptic Plasticity. Int J Radiat Oncol Biol Phys 112, 747-758.

Keppler, S.J., Goess, M.C., and Heinze, J.M. (2021). The Wanderings of Gut-Derived IgA Plasma Cells: Impact on Systemic Immune Responses. Front Immunol 12, 670290.

Hofmann, J., and Keppler, S.J. (2021). Tissue clearing and 3D imaging - putting immune cells into context. J Cell Sci 134.

Hofmann, J., Gadjalova, I., Mishra, R., Ruland, J., and Keppler, S.J. (2020). Efficient Tissue Clearing and Multi-Organ Volumetric Imaging Enable Quantitative Visualization of Sparse Immune Cell Populations During Inflammation. Front Immunol 11, 599495.

Keppler, S.J., Burbage, M., Gasparrini, F., Hartjes, L., Aggarwal, S., Massaad, M.J., Geha, R.S., Bruckbauer, A., and Batista, F.D. (2018). The Lack of WIP Binding to Actin Results in Impaired B Cell Migration and Altered Humoral Immune Responses. Cell Rep 24, 619-629.

Wartewig, T., Kurgyis, Z., Keppler, S., Pechloff, K., Hameister, E., Ollinger, R., Maresch, R., Buch, T., Steiger, K., Winter, C., et al. (2017). PD-1 is a haploinsufficient suppressor of T cell lymphomagenesis. Nature 552, 121-125.

Martinez-Martin, N., Maldonado, P., Gasparrini, F., Frederico, B., Aggarwal, S., Gaya, M., Tsui, C., Burbage, M., Keppler, S.J., Montaner, B., et al. (2017). A switch from canonical to noncanonical autophagy shapes B cell responses. Science 355, 641-647.

Keppler, S.J., Gasparrini, F., Burbage, M., Aggarwal, S., Frederico, B., Geha, R.S., Way, M., Bruckbauer, A., and Batista, F.D. (2015). Wiskott-Aldrich Syndrome Interacting Protein Deficiency Uncovers the Role of the Co-receptor CD19 as a Generic Hub for PI3 Kinase Signaling in B Cells. Immunity.

Keppler, S.J., and Aichele, P. (2011). Signal 3 requirement for memory CD8+ T-cell activation is determined by the infectious pathogen. Eur J Immunol 41, 3176-3186.

Keppler, S.J., Rosenits, K., Koegl, T., Vucikuja, S., and Aichele, P. (2012). Signal 3 cytokines as modulators of primary immune responses during infections: the interplay of type I IFN and IL-12 in CD8 T cell responses. PLoS One 7, e40865.

Dr. Selina Keppler
Technical University of Munich
School of Medicine
Institute for Clinical Chemistry and Pathobiochemistry
Tel: +49 89 4140 9255