Control and Manipulation of Microscale Living Objects

Ghulam Destgeer

Ghulam Destgeer studied mechanical engineering at GIK Institute, Pakistan (2010), and obtained his MS and PhD degrees in the field of acousto-microfluidics from KAIST, South Korea (2018). He conducted his postdoctoral research in the Di Carlo Lab at University of California, Los Angeles (UCLA). In November 2020, he joined TranslaTUM as a Tenure Track Assistant Professor in the Department of Electrical and Computer Engineering, where he leads the research group “Control and Manipulation of Microscale Living Objects”. Before that, he pioneered the acousto-microfluidic research at KAIST during his graduate studies, where he explored the use of travelling surface acoustic waves to efficiently manipulate micro-objects inside a microfluidic channel and a sessile droplet. At UCLA, he contributed to the development of newly envisioned ‘Lab on a Particle’ technology that uses amphiphilic, 3D structured and shape-coded particles to form uniform particle-templated droplets or ‘dropicles’ upon simple mixing of reagents. These ‘dropilces’ are used for amplified affinity based immunoassay or single cell/molecule analysis. He has coauthored more than 35 peer-reviewed articles in renowned journals of ‘Lab on a Chip’, ‘Analytical Chemistry’, ‘Advanced Science’, etc. He has presented his research work at more than 10 international platforms that include several invited talks.

Dr. Destgeer's (*1990) research focuses on the development of miniaturized microfluidic 'lab on a chip' platforms for efficient manipulation of micro-objects, including cells, particles, droplets, and microorganisms, using MHz-frequency acoustic waves. With a background in microfluidic platform development, he has a deep interest in handling the micro-objects (cell, particles, droplet, etc.) using passive as well as active microfluidic devices. Another research focus revolves around the development of newly envisioned 'lab on a particle' technology to fabricate 3D, multi-material, amphiphilic, shape-coded particles, and utilize them to spontaneously form 100s of particle-templated drops for single cell/molecule analysis within seconds. He hopes to use and further develop the ‘lab on a particle’ technology for the translational cancer research.

1.       G.Destgeer,* M.Ouyang,* C-Y.Wu and D.D.Carlo, "Fabrication of 3D concentric amphiphilic microparticles to form uniform nanoliter reaction volumes for amplified affinity assays", Lab on a Chip, 20, 3503-3514, (2020).

2.       M.Afzal,* J.Park,* G.Destgeer,* H.Ahmed, S.A.Iqrar, S.Kim, S.Kang, A.Alazzam, T.-S.Yoon and H.J.Sung, "Acousto-microfluidic Separation of Tardigrades from Raw Cultures for Sample Preparation", Zoological Journal of the Linnean Society, 188(3), 809–819, (2020).

3.       J.Park, G.Destgeer, M.Afzal and H.J.Sung, "Acoustofluidic Generation of Droplets with Tunable Chemical Concentrations", Lab on a Chip, 20, 3845-4084, (2020).

4.       G.Destgeer, A.Hashmi, J.Park, H.Ahmed, M.Afzal and H.J.Sung, "Microparticle Self-assembly Induced by Travelling Surface Acoustic Waves", RSC Advances, Vol. 9, pp.7916-7921, (2019).

5.       H.Ahmed,* J.Park,* G.Destgeer,* M.Afzal and H.J.Sung, "Surface Acoustic Wave-Based Micromixing Enhancement Using a Single Interdigital Transducer", Applied Physics Letters, Vol. 114, Issue 4, 043702, (2019).

6.       G.Destgeer, A. Alam, H. Ahmed, J.Park, J.H.Jung and H.J.Sung, "Characterization of Microchannel Anechoic Corners Formed by Surface Acoustic Waves", Applied Physics Letters, 112, 083501, (2018).

7.       J.H.Jung,* G.Destgeer,* J.Park, H.Ahmed, K.Park and H.J.Sung, "Microfluidic Flow Switching via Localized Acoustic Streaming Controlled by Surface Acoustic Waves", RSC Advances, 8, 3206-3212, (2018).

8.       J.Park, G.Destgeer, H.Kim, Y.Cho and H.J.Sung, " In-droplet microparticle washing and enrichment using surface acoustic wave-driven acoustic radiation force", Lab on a Chip, Vol. 18, Issue. 19, pp. 2936-2945, (2018).

9.       H.Ahmed, G.Destgeer, J.Park, M.Afzal and H.J.Sung, "Sheathless Focusing and Separation of Microparticles Using Tilted Angle Travelling Surface Acoustic Waves", Analytical Chemistry, 90, 14, 8546-8552, (2018).

10.   H.Ahmed, G.Destgeer, J.Park, J.H.Jung and H.J.Sung, "Vertical Hydrodynamic Focusing and Continuous Acoustofluidic Separation of Particles via Upward Migration", Advanced Science, 5 (2), 1700285, (2018).

11.   J.Park, J.H.Jung, K.Park, G.Destgeer, H.Ahmed, R.Ahmad and H.J.Sung, "On-demand acoustic droplet splitting and steering in a disposable microfluidic chip", Lab on a Chip, 18, 422-432, (2018).

12.   R.Ahmad,* G.Destgeer,* M.Afzal,* J.Park, H.Ahmed, H.Jung, K.Park, T.-S.Yoon and H.J.Sung, "Acoustic Wave-Driven Functionalized Particles for Aptamer-Based Target Biomolecule Separation", Analytical Chemistry, 89 (24), pp 13313–13319 (2017).

13.   G.Destgeer, J.H.Jung, J.Park, H.Ahmed, K.Park and H.J.Sung, "Acoustic impedance-based manipulation of elastic microspheres using travelling surface acoustic waves", RSC Advances, 7, 22524-22530, (2017).

14.   H.Ahmed, G.Destgeer, J.Park, J.H.Jung, R.Ahmad, K.Park and H.J.Sung, "A Pumpless Acoustofluidic Platform for Size-Selective Concentration and Separation of Microspheres", Analytical Chemistry, 89 (24), pp 13575–13581, (2017).

15.   J.Park, J.H.Jung, G.Destgeer, H.Ahmed, K.Park and H.J.Sung, "Acoustothermal tweezer for droplet sorting in a disposable microfluidic chip ", Lab on a Chip, 17, 1031-1040, (2017).

16.   J.H.Jung, G.Destgeer, J.Park, H.Ahmed, K.Park and H.J.Sung, "On-Demand Droplet Capture and Release Using Microwell-Assisted Surface Acoustic Waves", Analytical Chemistry, 89 (4), pp 2211–2215, (2017).

17.   K.Park, J.Park, J.H.Jung, G.Destgeer, H.Ahmed and H.J.Sung, "In-Droplet Microparticle Separation with Travelling Surface Acoustic Waves", Biomicrofluidics, 11 (6), 064112, (2017).

18.   G.Destgeer, J.H.Jung, J.Park, H.Ahmed and H.J.Sung, "Particle Separation inside a Sessile Droplet with Variable Contact Angle Using Surface Acoustic Waves", Analytical Chemistry, 89 (1), pp 736–744, (2016).

19.   G.Destgeer, A.Alazzam and H.J.Sung, "High Frequency Travelling Surface Acoustic Waves for Microparticle Separation", Journal of Mechanical Science and Technology, Vol.30, pp.3945-3952, (2016).

20.   G.Destgeer, B.H.Ha, J.Park and H.J.Sung, "Lamb Wave-Based Acoustic Radiation Force-Driven Particle Ring Formation inside a Sessile Droplet", Analytical Chemistry, 88 (7), pp.3976-3981, (2016).

21.   G.Destgeer, H.Cho, B.H.Ha, J.H.Jung, J.Park and H.J.Sung, "Acoustofluidic Particle Manipulation inside a Sessile Droplet: Four Distinct Modes of Particle Concentration", Lab on a Chip, Vol.16, pp.660-667, (2016).

22.   B.Mathew, A.Alazzam, G.Destgeer and H.J.Sung, "Dielectrophoresis based Cell Switching in Continuous Flow Microfluidic Devices", Journal of Electrostatics, Vol.84, pp.63-72, (2016).

23.   J.H.Jung, G.Destgeer, B.Ha, J.Park and H.J.Sung, "On-demand Droplet Splitting Using Surface Acoustic Waves", Lab on a Chip, Vol.16, pp.3235-3243, (2016).

24.   B.H.Ha, J.Park, G.Destgeer, J.H.Jung and H.J.Sung, "Transfer of Microparticles across Laminar Streams from Non-Newtonian to Newtonian Fluid", Analytical Chemistry, 88 (8), pp.4205-4210, (2016).

25.   J.Park, B.H.Ha, G.Destgeer, J.H.Jung and H.J.Sung, "Spatiotemporally Controllable Acoustothermal Heating and Its Application to Disposable Thermochromic Displays", RSC Advances, Vol.6, pp.33937-33944, (2016).

26.   J.H.Jung, K.S.Lee, S.Im, G.Destgeer, B.H.Ha, J.Park and H.J.Sung, "Photosynthesis of Cyanobacteria in a Miniaturized Optofluidic Waveguide Platform", RSC Advances, Vol.6, pp.11081-11087, (2016).

27.   G.Destgeer and H.J.Sung, "Recent Advances in Microfluidic Actuation and Micro-Object Manipulation via Surface Acoustic Waves", Lab on a Chip, Vol.15, pp.2722-2738, (2015).

28.   G.Destgeer, B.H.Ha, J.Park, J.H.Jung, A.Alazzam and H.J.Sung, "Microchannel Anechoic Corner for Size-Selective Separation and Medium Exchange via Travelling Surface Acoustic Waves", Analytical Chemistry, 87 (9), pp 4627–4632, (2015).

29.   B.H.Ha, J.Park, G.Destgeer, J.H.Jung and H.J.Sung, "Generation of Dynamic Free-Form Temperature Gradients in a Disposable Microchip", Analytical Chemistry, 87 (22), pp.11568-11574, (2015).

30.   B.Mathew, A.Alazzam, B.El-Khasawneh, M.Maalouf, G.Destgeer and H.J.Sung, "Model for Tracing the Path of Microparticles in Continuous Flow Microfluidic Devices for 2D Focusing via Standing Acoustic Waves", Journal of Separation and Purification Technology, Vol.153, pp.99-107, (2015).

31.   B.H.Ha, K.S.Lee, G.Destgeer, J.Park, J.S.Choung, J.H.Jung, J.H.Shin and H.J.Sung, "Acoustothermal heating of polydimethylsiloxane microfluidic system", Scientific Reports, 5, 11851, (2015).

32.   J.H.Jung, K.H.Lee, G.Destgeer, K.S.Lee, H.Cho, B.H.Ha and H.J.Sung, "In Situ, Seriate Droplet Coalescence under an Optical Force", Microfluidics and Nanofluidics, Vol.18, Issue 5, pp.1247-1254, (2015).

33.   G.Destgeer, B.H.Ha, J.H.Jung and H.J.Sung, "Submicron Separation of Microspheres via Travelling Surface Acoustic Waves", Lab on a Chip, Vol.14, pp.4665-4672, (2014).

34.   G.Destgeer, S.Im, B.H.Ha, J.H.Jung, M.A.Ansari and H.J.Sung, "Adjustable, Rapidly Switching Microfluidic Gradient Generation Using Travelling Surface Acoustic Waves", Applied Physics Letters, Vol.104, Issue 21, 023506, (2014).

35.   G.Destgeer, K.H.Lee, J.H.Jung, A.Alazzam and H.J.Sung, "Continuous Separation of Particles in a PDMS Microfluidic Channel via Travelling Surface Waves (TSAW)", Lab on a Chip, Vol.13, pp.4210-4216, (2013).